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YFP NLS Beta-Actin G13R
(Plasmid #60615)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60615 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 60615-D50 50 μg of DNA in Tris buffer $495
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DNA 60615-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEYFP-C1
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5800
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Beta-actin
  • Species
    H. sapiens (human)
  • Mutation
    G13R
  • GenBank ID
    NM_001101.3
  • Entrez Gene
    ACTB (a.k.a. BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8)
  • Tags / Fusion Proteins
    • EYFP (N terminal on insert)
    • NLS (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Derived from Clonetech pEYFP-Actin

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YFP NLS Beta-Actin G13R was a gift from Primal de Lanerolle (Addgene plasmid # 60615 ; http://n2t.net/addgene:60615 ; RRID:Addgene_60615)

  • For your References section:

    Herpesviral replication compartments move and coalesce at nuclear speckles to enhance export of viral late mRNA. Chang L, Godinez WJ, Kim IH, Tektonidis M, de Lanerolle P, Eils R, Rohr K, Knipe DM. Proc Natl Acad Sci U S A. 2011 May 24;108(21):E136-44. doi: 10.1073/pnas.1103411108. Epub 2011 May 9. 10.1073/pnas.1103411108 PubMed 21555562
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