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pmCherry-C1 R62D actin-3XNLS P2A mCherry
(Plasmid #58477)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 58477 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    mCherry-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5994
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    beta actin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1125
  • Mutation
    Changed Arginine 62 to Aspartic Acid
  • GenBank ID
    NM_001101.3
  • Promoter CMV

Cloning Information for Gene/Insert 1

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer mCherry Reverse
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    3XNLS
  • Alt name
    nuclear localization signal
  • Species
    SV40 virus
  • Insert Size (bp)
    72
  • Promoter CMV

Cloning Information for Gene/Insert 2

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer actin Forward
  • 3′ sequencing primer mCherry Reverse
  • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    P2A cleavage site
  • Alt name
    2A Peptide Derived from Porcine Teschovirus-1
  • Species
    Porcine Teschovirus-1
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information for Gene/Insert 3

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer actin Forward
  • 3′ sequencing primer mCherry Reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    P2A peptide and 3X NLS (with stop codons) were inserted into the pmCherry-C1 backbone via GenScript's custom cloning services. Human actin was cloned from cDNA using Gibson cloning into an NLS-P2A-mCherry backbone.
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmCherry-C1 R62D actin-3XNLS P2A mCherry was a gift from Dyche Mullins (Addgene plasmid # 58477)
  • For your References section:

    DNA damage induces nuclear actin filament assembly by formin-2 and Spire-1/2 that promotes efficient DNA repair. Belin BJ, Lee T, Mullins RD. Elife. 2015 Aug 19;4. doi: 10.7554/eLife.07735. 10.7554/eLife.07735 PubMed 26287480