Zf Slit1a
(Plasmid
#61279)
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Purposecontains zebrafish Slit1a cDNA
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Depositing Labs
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61279 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCRII-TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3950
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Vector typein vitro transcription
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSlit1a
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SpeciesD. rerio (zebrafish)
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Entrez Geneslit1a (a.k.a. CH211-178H7.3)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
antisense probe: cut with XhoI or NotI and use SP6
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Zf Slit1a was a gift from Chi-Bin Chien & Lara Hutson (Addgene plasmid # 61279 ; http://n2t.net/addgene:61279 ; RRID:Addgene_61279) -
For your References section:
Two divergent slit1 genes in zebrafish. Hutson LD, Jurynec MJ, Yeo SY, Okamoto H, Chien CB. Dev Dyn. 2003 Nov;228(3):358-69. 10.1002/dvdy.10386 PubMed 14579375