Zf Slit1b
(Plasmid
#61280)
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Purposecontains zebrafish Slit1b cDNA
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Depositing Labs
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61280 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBSII SK(+)
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Backbone manufacturerStratagene
- Total vector size (bp) 8100
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Vector typecloning vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSlit1b
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SpeciesD. rerio (zebrafish)
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Entrez Geneslit1b
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer M13pUC-Rev; T3
- 3′ sequencing primer M13pUC-Fwd; T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
antisense probe: cut with SmaI and use T7.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Zf Slit1b was a gift from Chi-Bin Chien & Lara Hutson (Addgene plasmid # 61280 ; http://n2t.net/addgene:61280 ; RRID:Addgene_61280) -
For your References section:
Two divergent slit1 genes in zebrafish. Hutson LD, Jurynec MJ, Yeo SY, Okamoto H, Chien CB. Dev Dyn. 2003 Nov;228(3):358-69. 10.1002/dvdy.10386 PubMed 14579375