PurposeExpresses calmodulin binding protein-LovTAP N538E fusion protein in E. coli cells. LovTAP N358E is a optogenetic protein that can repress the Trp promoter in the presence of blue light.
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61431||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Gene/Insert nameLovTAP N538E
Insert Size (bp)465
- Promoter T7 lac operator
/ Fusion Protein
- Cloning method Unknown
- 5′ sequencing primer T7 short
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LovTAP N538E was a gift from Tobin Sosnick (Addgene plasmid # 61431 ; http://n2t.net/addgene:61431 ; RRID:Addgene_61431)
For your References section:Rationally improving LOV domain-based photoswitches. Strickland D, Yao X, Gawlak G, Rosen MK, Gardner KH, Sosnick TR. Nat Methods. 2010 Aug;7(8):623-6. doi: 10.1038/nmeth.1473. Epub 2010 Jun 20. 10.1038/nmeth.1473 PubMed 20562867