Skip to main content
Addgene
Open Menu

pERB215
(Plasmid #61499)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61499 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 61499-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 61499-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pEM791
  • Backbone manufacturer
    Dr. Eugene Makeyev
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    HaloTag-GFP-Nuf2
  • Species
    H. sapiens (human)
  • Tag / Fusion Protein
    • HaloTag (N terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    mCherry-DHFR
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pERB215 was a gift from David Chenoweth & Michael Lampson (Addgene plasmid # 61499 ; http://n2t.net/addgene:61499 ; RRID:Addgene_61499)

  • For your References section:

    Localized light-induced protein dimerization in living cells using a photocaged dimerizer. Ballister ER, Aonbangkhen C, Mayo AM, Lampson MA, Chenoweth DM. Nat Commun. 2014 Nov 17;5:5475. doi: 10.1038/ncomms6475. 10.1038/ncomms6475 PubMed 25400104
Related items:
Commonly requested with: