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Beta-2-adrenergic receptor-mCerulean
(Plasmid #66957)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 66957 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Derived from pECFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3954
  • Total vector size (bp) 5967
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Beta-2-adrenergic receptor-mCerulean
  • Alt name
    ADRB2
  • Species
    H. sapiens (human); Aequorea victoria
  • Insert Size (bp)
    2013
  • Entrez Gene
    ADRB2 (a.k.a. ADRB2R, ADRBR, B2AR, BAR, BETA2AR)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCerulean (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Beta-2-adrenergic receptor-GFP was obtained from Gerda Breitweiser, Weis Center for Research, Geisinger Clinic, Danville, PA. Monomeric Cerulean was obtained from David Piston, Vanderbilt University, Nashville, TN.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Reference for monomeric Cerulean:

Rizzo MA, Springer GH, Granada B, Piston DW. An improved cyan fluorescent protein variant useful for FRET. Nat Biotechnol. 2004;22(4):445-9. PubMed PMID: 14990965.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Beta-2-adrenergic receptor-mCerulean was a gift from Catherine Berlot (Addgene plasmid # 66957 ; http://n2t.net/addgene:66957 ; RRID:Addgene_66957)
  • For your References section:

    Live cell imaging of Gs and the beta2-adrenergic receptor demonstrates that both alphas and beta1gamma7 internalize upon stimulation and exhibit similar trafficking patterns that differ from that of the beta2-adrenergic receptor. Hynes TR, Mervine SM, Yost EA, Sabo JL, Berlot CH. J Biol Chem. 2004 Oct 15;279(42):44101-12. Epub 2004 Aug 5. 10.1074/jbc.M405151200 PubMed 15297467