PurposeBeta-2-adrenergic receptor tagged with mCerulean on carboxyl terminus
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||66957||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backboneDerived from pECFP-N1
- Backbone size w/o insert (bp) 3954
- Total vector size (bp) 5967
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert nameBeta-2-adrenergic receptor-mCerulean
SpeciesH. sapiens (human); Aequorea victoria
Insert Size (bp)2013
Entrez GeneADRB2 (a.k.a. ADRB2R, ADRBR, B2AR, BAR, BETA2AR)
- Promoter CMV
/ Fusion Protein
- mCerulean (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byBeta-2-adrenergic receptor-GFP was obtained from Gerda Breitweiser, Weis Center for Research, Geisinger Clinic, Danville, PA. Monomeric Cerulean was obtained from David Piston, Vanderbilt University, Nashville, TN.
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Reference for monomeric Cerulean:
Rizzo MA, Springer GH, Granada B, Piston DW. An improved cyan fluorescent protein variant useful for FRET. Nat Biotechnol. 2004;22(4):445-9. PubMed PMID: 14990965.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Beta-2-adrenergic receptor-mCerulean was a gift from Catherine Berlot (Addgene plasmid # 66957 ; http://n2t.net/addgene:66957 ; RRID:Addgene_66957)
For your References section:Live cell imaging of Gs and the beta2-adrenergic receptor demonstrates that both alphas and beta1gamma7 internalize upon stimulation and exhibit similar trafficking patterns that differ from that of the beta2-adrenergic receptor. Hynes TR, Mervine SM, Yost EA, Sabo JL, Berlot CH. J Biol Chem. 2004 Oct 15;279(42):44101-12. Epub 2004 Aug 5. 10.1074/jbc.M405151200 PubMed 15297467