pCMV-DnpK
(Plasmid #71403)

• Purpose: Expresses tRNA and synthetases for mammalian encoding of a dinitrophenyl hapten, DnpK
• Depositing Lab: Huiwang Ai
• Publication: Ren et al Chembiochem. 2015 Sep;16(14):2007-10. doi: 10.1002/cbic.201500204. Epub 2015 Jul 28.

Backbone

• Vector backbone: unknown
• Total vector size (bp): 7245
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: aminoacyl-tRNA synthetase
• Species: Synthetic
• Insert Size (bp): 1200
• Mutation: Y271M,L274T,C313A, and Y349F
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: pCMV

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The pyrrolysyl-tRNA-synthetase insert contains Y272M, L275T, C314A and Y350Fcompared to the closest reference sequence ACB70968.1. The depositor states that these mutations are intended.