PurposeExpresses tRNA and synthetases for mammalian encoding of a dinitrophenyl hapten, DnpK
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||71403||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 7245
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameaminoacyl-tRNA synthetase
Insert Size (bp)1200
MutationY271M,L274T,C313A, and Y349F
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer pCMV (Common Sequencing Primers)
The pyrrolysyl-tRNA-synthetase insert contains Y272M, L275T, C314A and Y350Fcompared to the closest reference sequence ACB70968.1. The depositor states that these mutations are intended.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-DnpK was a gift from Huiwang Ai (Addgene plasmid # 71403 ; http://n2t.net/addgene:71403 ; RRID:Addgene_71403)
For your References section:Expanding the Genetic Code for a Dinitrophenyl Hapten. Ren W, Ji A, Wang MX, Ai HW. Chembiochem. 2015 Sep;16(14):2007-10. doi: 10.1002/cbic.201500204. Epub 2015 Jul 28. 10.1002/cbic.201500204 PubMed 26185102
Map uploaded by the depositor.