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(Plasmid #64915)


Item Catalog # Description Quantity Price (USD)
Plasmid 64915 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 8244
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Polyspecific aminoacyl-tRNA Synthetase
  • Alt name
    E. coli tyrosyl synthetase with Y37I/D182S/F183M/D265R mutations
  • Species
  • Insert Size (bp)
  • Promoter CMV
  • Tag / Fusion Protein
    • None

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site PmeI or ApaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-R
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Amber Suppression tRNA
  • Species
  • Promoter U6/H1

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer tRNA-F
  • 3′ sequencing primer tRNA-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please cite Z. Chen, W. Ren, Q.E. Wright and H-w. Ai*, J. Am. Chem. Soc., 2013, 135: 14940-14943.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMAH-POLY was a gift from Huiwang Ai (Addgene plasmid # 64915 ; ; RRID:Addgene_64915)
  • For your References section:

    Genetically encoded fluorescent probe for the selective detection of peroxynitrite. Chen ZJ, Ren W, Wright QE, Ai HW. J Am Chem Soc. 2013 Oct 9;135(40):14940-3. doi: 10.1021/ja408011q. Epub 2013 Sep 26. 10.1021/ja408011q PubMed 24059533