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pcDNA3-hsGFP
(Plasmid #64912)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 64912 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5968
  • Total vector size (bp) 6748
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hsGFP
  • Species
    Synthetic
  • Insert Size (bp)
    780
  • Mutation
    Chromophore tyrosine of the protein mutated to TAG amber codon
  • Promoter CMV
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pCMV-F
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please cite Z. Chen and H-w. Ai*, Biochemistry, 2014, 53: 5966-5974; and S. Chen, Z. Chen, W. Ren and H-w. Ai*, J. Am. Chem. Soc., 2012, 134: 9589-9592. Please note that you will also need a pMAH plasmid for mammalian expression. pMAH-POLY (Z. Chen, W. Ren, Q.E. Wright and H-w. Ai*, J. Am. Chem. Soc., 2013, 135: 14940-14943.) is also deposited to Addgene, which can be used to replace pMAH-AzF for mammalian applications.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-hsGFP was a gift from Huiwang Ai (Addgene plasmid # 64912 ; http://n2t.net/addgene:64912 ; RRID:Addgene_64912)
  • For your References section:

    A highly responsive and selective fluorescent probe for imaging physiological hydrogen sulfide. Chen ZJ, Ai HW. Biochemistry. 2014 Sep 23;53(37):5966-74. doi: 10.1021/bi500830d. Epub 2014 Sep 5. 10.1021/bi500830d PubMed 25141269