PurposeEncodes a Fluorescent Reporter for Hydrogen Sulfide in Mammalian Cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||64912||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5968
- Total vector size (bp) 6748
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Insert Size (bp)780
MutationChromophore tyrosine of the protein mutated to TAG amber codon
- Promoter CMV
/ Fusion Protein
- 6xHis (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pCMV-F
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please cite Z. Chen and H-w. Ai*, Biochemistry, 2014, 53: 5966-5974; and S. Chen, Z. Chen, W. Ren and H-w. Ai*, J. Am. Chem. Soc., 2012, 134: 9589-9592. Please note that you will also need a pMAH plasmid for mammalian expression. pMAH-POLY (Z. Chen, W. Ren, Q.E. Wright and H-w. Ai*, J. Am. Chem. Soc., 2013, 135: 14940-14943.) is also deposited to Addgene, which can be used to replace pMAH-AzF for mammalian applications.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-hsGFP was a gift from Huiwang Ai (Addgene plasmid # 64912 ; http://n2t.net/addgene:64912 ; RRID:Addgene_64912)
For your References section:A highly responsive and selective fluorescent probe for imaging physiological hydrogen sulfide. Chen ZJ, Ai HW. Biochemistry. 2014 Sep 23;53(37):5966-74. doi: 10.1021/bi500830d. Epub 2014 Sep 5. 10.1021/bi500830d PubMed 25141269