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pcDNA3-TrxRFP1
(Plasmid #98996)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 98996 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human thioredoxin 1, GS-rich linker, fused with a redox-sensitive RFP
  • Alt name
    TrxRFP1
  • Species
    Synthetic
  • Insert Size (bp)
    1311
  • GenBank ID
    KX981912
  • Promoter CMV
  • Tag / Fusion Protein
    • His-tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV forward
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-TrxRFP1 was a gift from Huiwang Ai (Addgene plasmid # 98996 ; http://n2t.net/addgene:98996 ; RRID:Addgene_98996)
  • For your References section:

    Monitoring thioredoxin redox with a genetically encoded red fluorescent biosensor. Fan Y, Makar M, Wang MX, Ai HW. Nat Chem Biol. 2017 Jun 26. doi: 10.1038/nchembio.2417. 10.1038/nchembio.2417 PubMed 28671680