pCXLE-hOCT3/4-shp53-F + mCherry-2A-puro
(Plasmid #74947)

• Purpose: Dosage control and tracing of reprogramming episomes
• Depositing Lab: Ann Zovein
• Publication: Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132. doi: 10.1186/s13287-017-0581-7.

Backbone

• Vector backbone: pCXLE
• Total vector size (bp): 14800
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Oct3/4
• Species: H. sapiens (human)
• Entrez Gene: POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: FseI (unknown if destroyed)
• 3′ cloning site: AsiSI (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry