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RP4-7
(Plasmid #79811)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 79811 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 79811-D50 50 μg of DNA in Tris buffer $495
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DNA 79811-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    RP4
  • Backbone manufacturer
    natural plasmid
  • Backbone size w/o insert (bp) 60000
  • Total vector size (bp) 60002
  • Modifications to backbone
    Insertion of Cm cassette by in vivo recombination. For details see original Quandt et al. paper from 2004.
  • Vector type
    Helper plasmid for mobilization

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    CSH56
  • Growth instructions
    Select for the chromosome with nalidixic acid.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Chloramphenicol acetyltransferase
  • Alt name
    Cm resistance
  • Species
    E. coli
  • Promoter Own promoter

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We made this plasmid by in vivo recombination. See details in original paper.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor states that users can select for the chromosome with nalidixic acid.

Depositor states that growth on the respective antibiotic is sufficient to confirm plasmid integrity

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RP4-7 was a gift from Michael Hynes (Addgene plasmid # 79811 ; http://n2t.net/addgene:79811 ; RRID:Addgene_79811)

  • For your References section:

    Modified RP4 and Tn5-Mob derivatives for facilitated manipulation of large plasmids in Gram-negative bacteria. Quandt J, Clark RG, Venter AP, Clark SR, Twelker S, Hynes MF. Plasmid. 2004 Jul;52(1):1-12. 10.1016/j.plasmid.2004.04.002 PubMed 15212888
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