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Addgene

RP4-11
(Plasmid #79812)

Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 79812 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    RP4
  • Backbone manufacturer
    natural plasmid
  • Backbone size w/o insert (bp) 60000
  • Total vector size (bp) 60004
  • Modifications to backbone
    Insertion of KmSacB cassette by in vivo recombination. For details see original Quandt et al. paper from 2004.
  • Vector type
    Helper plasmid for mobilization

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    CSH56
  • Growth instructions
    Select for the chromosome with nalidixic acid.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Kanamycin cassette and SacB gene
  • Alt name
    Km, SacB
  • Insert Size (bp)
    2800
  • Entrez Gene
    sacB (a.k.a. BSU_34450, BSU34450)
  • Promoter Km

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We made this plasmid by in vivo recombination

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is designed to be used in mobilizing other plasmids, and is easily curable from recipient cells because of the sacB gene. Depositor states that growth on the respective antibiotic is sufficient to confirm plasmid integrity.

Depositor states that users can select for the chromosome with nalidixic acid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RP4-11 was a gift from Michael Hynes (Addgene plasmid # 79812 ; http://n2t.net/addgene:79812 ; RRID:Addgene_79812)
  • For your References section:

    Modified RP4 and Tn5-Mob derivatives for facilitated manipulation of large plasmids in Gram-negative bacteria. Quandt J, Clark RG, Venter AP, Clark SR, Twelker S, Hynes MF. Plasmid. 2004 Jul;52(1):1-12. 10.1016/j.plasmid.2004.04.002 PubMed 15212888