Purpose(Empty Backbone) Shuttle vector suitable for mobilizing plasmids to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strains.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||70261||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonederived from RP4
Modifications to backbonederived from RP4, which is identical, or nearly so, to Birmingham IncP-alpha plasmid isolate RK2, whose sequence is presented in GenBank: BN000925.1
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberLow Copy
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Because many plasmids that are mobilized to cyanobacteria by RP4 use kanamycin for selection, it is convenient to have RP4 not retain kanamycin-resistance. pRL443 is an RP4-mutant that reverts to kanamycin resistance with a frequency of about 3x10-9, consistent with the lesion being a point mutation (Elhai et al., J Bacteriol 179: 1998-2005, 1997).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRL443 was a gift from Peter Wolk (Addgene plasmid # 70261 ; http://n2t.net/addgene:70261 ; RRID:Addgene_70261)
For your References section:Reduction of conjugal transfer efficiency by three restriction activities of Anabaena sp. strain PCC 7120. Elhai J, Vepritskiy A, Muro-Pastor AM, Flores E, Wolk CP. J Bacteriol. 1997 Mar;179(6):1998-2005. PubMed 9068647