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pRL443
(Plasmid #70261)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 70261 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    derived from RP4
  • Modifications to backbone
    derived from RP4, which is identical, or nearly so, to Birmingham IncP-alpha plasmid isolate RK2, whose sequence is presented in GenBank: BN000925.1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Resource Information

  • Terms and Licenses

Depositor Comments

Because many plasmids that are mobilized to cyanobacteria by RP4 use kanamycin for selection, it is convenient to have RP4 not retain kanamycin-resistance. pRL443 is an RP4-mutant that reverts to kanamycin resistance with a frequency of about 3x10-9, consistent with the lesion being a point mutation (Elhai et al., J Bacteriol 179: 1998-2005, 1997).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRL443 was a gift from Peter Wolk (Addgene plasmid # 70261 ; http://n2t.net/addgene:70261 ; RRID:Addgene_70261)
  • For your References section:

    Reduction of conjugal transfer efficiency by three restriction activities of Anabaena sp. strain PCC 7120. Elhai J, Vepritskiy A, Muro-Pastor AM, Flores E, Wolk CP. J Bacteriol. 1997 Mar;179(6):1998-2005. PubMed 9068647