Purposehelper plasmid for bacterial conjugal DNA transfer
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||58494||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Growth in Bacteria
Gene/Insert nameencodes methylases AvaiM, Eco47iiM, and Ecot22iM that target sites subject to restriction in Anabaena sp. strain PCC 7120
Alt nameMobColK, M.AvaI, M.Eco47II, M.EcoT22I
MobColK, M.AvaI, M.Eco47II, M.EcoT22I
Please see attached map for more information on how this vector was constructed and cloning details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRL623 was a gift from Peter Wolk (Addgene plasmid # 58494 ; http://n2t.net/addgene:58494 ; RRID:Addgene_58494)
For your References section:Reduction of conjugal transfer efficiency by three restriction activities of Anabaena sp. strain PCC 7120. Elhai J, Vepritskiy A, Muro-Pastor AM, Flores E, Wolk CP. J Bacteriol. 1997 Mar;179(6):1998-2005. PubMed 9068647