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pJ3 SHP2 E76A
(Plasmid #8321)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8321 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 8321-D50 50 μg of DNA in Tris buffer $495
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DNA 8321-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pJ3omega
  • Backbone size w/o insert (bp) 3500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SHP2
  • Alt name
    SH-PTP2
  • Alt name
    PTPN11
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2276
  • Mutation
    E76A (activated mutant)
  • GenBank ID
    L03535
  • Entrez Gene
    PTPN11 (a.k.a. BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The SHP2 E76A mutation is described in "Activated mutants of SHP-2 preferentially induce elongation of Xenopus animal caps" O'Reilly AM, Pluskey S, Shoelson SE, Neel BG. Mol Cell Biol. 2000 Jan;20(1):299-311. PubMed ID: 10594032

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJ3 SHP2 E76A was a gift from Ben Neel (Addgene plasmid # 8321 ; http://n2t.net/addgene:8321 ; RRID:Addgene_8321)

  • For your References section:

    Multiple requirements for SHPTP2 in epidermal growth factor-mediated cell cycle progression. Bennett AM, Hausdorff SF, O'Reilly AM, Freeman RM, Neel BG. Mol Cell Biol 1996 Mar;16(3):1189-202. PubMed 8622663
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