hum sgk promoter F4R4
(Plasmid
#83426)
-
Purposepromoter reporter construct to test 5' flanking sequences
-
Depositing Lab
-
Depositing OrganizationUniversity of Iowa
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 83426 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 83426-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 83426-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepGL3basic
-
Backbone manufacturerPromega
-
Vector typeLuciferase
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namehuman Sgk1 gene 5' flanking seq + 117 nt of the 5' UTR
-
Alt nameSgk1F4R4
-
SpeciesH. sapiens (human)
-
Insert Size (bp)3259
-
Entrez GeneSGK1 (a.k.a. SGK)
- Promoter sgk
-
Tag
/ Fusion Protein
- Luciferase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNRev
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 83426-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 83426-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
hum sgk promoter F4R4 was a gift from Christie Thomas (Addgene plasmid # 83426 ; http://n2t.net/addgene:83426 ; RRID:Addgene_83426) -
For your References section:
Glucocorticoids stimulate human sgk1 gene expression by activation of a GRE in its 5'-flanking region. Itani OA, Liu KZ, Cornish KL, Campbell JR, Thomas CP. Am J Physiol Endocrinol Metab. 2002 Nov;283(5):E971-9. 10.1152/ajpendo.00021.2002 PubMed 12376324
