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hsgk1
(Plasmid #83432)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 83432 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human Sgk1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1352
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hsgk1 was a gift from Christie Thomas (Addgene plasmid # 83432 ; http://n2t.net/addgene:83432 ; RRID:Addgene_83432)
  • For your References section:

    An evolutionarily conserved N-terminal Sgk1 variant with enhanced stability and improved function. Raikwar NS, Snyder PM, Thomas CP. Am J Physiol Renal Physiol. 2008 Nov;295(5):F1440-8. doi: 10.1152/ajprenal.90239.2008. Epub 2008 Aug 27. 10.1152/ajprenal.90239.2008 PubMed 18753299