myc-Rictor corrected
(Plasmid #11367)

• Purpose: Mammalian expression of myc tagged human RICTOR
• Depositing Lab: David Sabatini
• Publication: Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Backbone

• Vector backbone: pRK-5
• Backbone manufacturer: BD Pharmingen
• Backbone size w/o insert (bp): 4754
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rictor
• Species: H. sapiens (human)
• Insert Size (bp): 5700
• Entrez Gene: RICTOR (a.k.a. AVO3, PIA, hAVO3)
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: SP6

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 31 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The amino acid sequence of this plasmid matches the NCBI published sequence. No functional differences between the plasmid with the point mutations (Addgene #1860) and this plasmid have been observed thus far. In this version, silent point mutations in the N-terminus were created to destroy the NotI site and facilitate cloning. These will not change the amino acid sequence of the protein. The ORF sequence of the new insert has been uploaded and is linked from this datasheet.