PurposeMammalian expression of myc tagged human RICTOR
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11367||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerBD Pharmingen
- Backbone size w/o insert (bp) 4754
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)5700
Entrez GeneRICTOR (a.k.a. AVO3, PIA, hAVO3)
/ Fusion Protein
- Myc (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
The amino acid sequence of this plasmid matches the NCBI published sequence. No functional differences between the plasmid with the point mutations (Addgene #1860) and this plasmid have been observed thus far. In this version, silent point mutations in the N-terminus were created to destroy the NotI site and facilitate cloning. These will not change the amino acid sequence of the protein. The ORF sequence of the new insert has been uploaded and is linked from this datasheet.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:myc-Rictor corrected was a gift from David Sabatini (Addgene plasmid # 11367 ; http://n2t.net/addgene:11367 ; RRID:Addgene_11367)
For your References section:Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Sarbassov DD, Ali SM, Kim DH, Guertin DA, Latek RR, Erdjument-Bromage H, Tempst P, Sabatini DM. Curr Biol 2004 Jul 27;14(14):1296-302. 10.1016/j.cub.2004.06.054 PubMed 15268862