myc-Raptor
(Plasmid #1859)

• Depositing Lab: David Sabatini
• Publication: Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Backbone

• Vector backbone: pRK-5
  (Search Vector Database)
• Backbone manufacturer: BD Pharmingen
• Backbone size w/o insert (bp): 4754
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Raptor
• Species: H. sapiens (human)
• Insert Size (bp): 6000
• Entrez Gene: RPTOR (a.k.a. KOG1, Mip1)
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 16 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry