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myc-Rictor
(Plasmid #1860)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 1860 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRK-5
  • Backbone manufacturer
    BD Pharmingen
  • Backbone size w/o insert (bp) 4754
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rictor
  • Species
    H. sapiens (human)
  • Entrez Gene
    RICTOR (a.k.a. AVO3, PIA, hAVO3)
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site XmaI (not destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Please note: This plasmid contains 2 amino acids that are different than the published NCBI sequence. The changes can be viewed in the Addgene Full Sequence link above- T->C at bp 1312 resulting in amino acid 35 being changed from S->P and a T->C change at bp 1556 resulting in amino acid 116 being changed from I->T. A new version of this plasmid with the corrected sequence is available (Addgene #11367).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    myc-Rictor was a gift from David Sabatini (Addgene plasmid # 1860 ; http://n2t.net/addgene:1860 ; RRID:Addgene_1860)
  • For your References section:

    Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Sarbassov DD, Ali SM, Kim DH, Guertin DA, Latek RR, Erdjument-Bromage H, Tempst P, Sabatini DM. Curr Biol 2004 Jul 27;14(14):1296-302. 10.1016/j.cub.2004.06.054 PubMed 15268862