myc mTOR kinase dead
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8482||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4716
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesR. norvegicus (rat)
Insert Size (bp)4500
Entrez GeneMtor (a.k.a. Frap1, RAFT1)
/ Fusion Protein
- myc (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site sal (unknown if destroyed)
- 3′ cloning site not (unknown if destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
NOTE: Though the depositors list SalI and NotI as the 5' and 3' cloning sites, respectively, Addgene's quality control sequence shows that there is actually only a SalI at the 3' end of the insert. There is no NotI present, so it may have been destroyed. Please refer to Addgene's sequence for further sub-cloning options.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:myc mTOR kinase dead was a gift from David Sabatini (Addgene plasmid # 8482 ; http://n2t.net/addgene:8482 ; RRID:Addgene_8482)