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hSyn1-sypHy-RGECO
(Plasmid #84078)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84078 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 84078-D50 50 μg of DNA in Tris buffer $495
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DNA 84078-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEGFP-N2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3903
  • Total vector size (bp) 6846
  • Modifications to backbone
    CMV promoter replaced with hSyn1 promoter, EGFP removed.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sypHy-RGECO
  • Alt name
    Rat Synaptophysin-pHluorin and R-GECO1
  • Species
    R. norvegicus (rat), Synthetic
  • Insert Size (bp)
    2943
  • Promoter hSyn1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer ACTCAGCGCTGCCTCAGTC
  • 3′ sequencing primer TTATGTTTCAGGTTCAGGG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    SypHy was subcloned from CMV-SypHy A4 (Addgene 24478) and R-GECO1 from CMV-R-GECO1 (Addgene 32444)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hSyn1-sypHy-RGECO was a gift from Juan Burrone (Addgene plasmid # 84078 ; http://n2t.net/addgene:84078 ; RRID:Addgene_84078)

  • For your References section:

    Visualizing Presynaptic Calcium Dynamics and Vesicle Fusion with a Single Genetically Encoded Reporter at Individual Synapses. Jackson RE, Burrone J. Front Synaptic Neurosci. 2016 Jul 26;8:21. doi: 10.3389/fnsyn.2016.00021. eCollection 2016. 10.3389/fnsyn.2016.00021 PubMed 27507942
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