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(Plasmid #23999)


Item Catalog # Description Quantity Price (USD)
Plasmid 23999 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
    NMDA 1 isoform 1a precursor
  • Species
    R. norvegicus (rat)
  • GenBank ID
    NM_017010.2 NP_058706.1
  • Entrez Gene
    Grin1 (a.k.a. GluN1, NMDAR1, NR1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • NR1 signal sequence (aa1-20) (N terminal on backbone)
    • SEP (superecliptic pHluorin) (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The super-ecliptic pHluorin (SEP) coding sequence was inserted three amino acids downstream of the predicted signal peptide cleavage site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-SEP-NRI was a gift from Robert Malinow (Addgene plasmid # 23999 ; ; RRID:Addgene_23999)
  • For your References section:

    Glutamate receptor exocytosis and spine enlargement during chemically induced long-term potentiation. Kopec CD, Li B, Wei W, Boehm J, Malinow R. J Neurosci. 2006 Feb 15. 26(7):2000-9. 10.1523/JNEUROSCI.3918-05.2006 PubMed 16481433

Ready-to-use antibodies

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