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pCI-SEP_NR2B
(Plasmid #23998)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 23998 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    JM109
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NR2B
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    6300
  • GenBank ID
    EDM01617.1
  • Entrez Gene
    Grin2b (a.k.a. GluN2B)
  • Promoter CMV
  • Tags / Fusion Proteins
    • NR2B signal sequence (aa1-31) (N terminal on backbone)
    • SEP (superecliptic pHluorin) (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

See attached map for additional plasmid features.
The super-ecliptic pHluorin (SEP) coding sequence was inserted three amino acids downstream of the predicted signal peptide cleavage site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-SEP_NR2B was a gift from Robert Malinow (Addgene plasmid # 23998 ; http://n2t.net/addgene:23998 ; RRID:Addgene_23998)
  • For your References section:

    Glutamate receptor exocytosis and spine enlargement during chemically induced long-term potentiation. Kopec CD, Li B, Wei W, Boehm J, Malinow R. J Neurosci. 2006 Feb 15. 26(7):2000-9. 10.1523/JNEUROSCI.3918-05.2006 PubMed 16481433