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pLCNICK
(Plasmid #84653)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84653 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 84653-D50 50 μg of DNA in Tris buffer $495
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DNA 84653-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCas
  • Backbone manufacturer
    Professor Sheng Yang (Plasmid 62225)
  • Backbone size w/o insert (bp) 4500
  • Total vector size (bp) 14300
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    Cas9 nickase
  • Species
    S. pyogenes
  • Insert Size (bp)
    4107
  • Mutation
    D10A
  • GenBank ID
    AE004092

Gene/Insert 2

  • Gene/Insert name
    sgRNA
  • Insert Size (bp)
    103

Gene/Insert 3

  • Gene/Insert name
    homology arms of LC2W_2179
  • Insert Size (bp)
    2007

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLCNICK was a gift from Sheng Yang (Addgene plasmid # 84653 ; http://n2t.net/addgene:84653 ; RRID:Addgene_84653)

  • For your References section:

    CRISPR-Cas9D10A Nickase-Assisted Genome Editing in Lactobacillus casei. Song X, Huang H, Xiong Z, Ai L, Yang S. Appl Environ Microbiol. 2017 Sep 1. pii: AEM.01259-17. doi: 10.1128/AEM.01259-17. 10.1128/AEM.01259-17 PubMed 28864652
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