PurposeFor Flpe/FRT-based Supernova, pK036 should be used with pK068 etc.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||85007||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2581
- Total vector size (bp) 4572
Vector typeMammalian Expression
Growth in Bacteria
SpeciesS. cerevisiae (budding yeast); SV40, Woodchuck hepatitis virus
Insert Size (bp)1991
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer LNCX_primer (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pK036.TRE-Flpe-WPRE (Supernova) was a gift from Takuji Iwasato (Addgene plasmid # 85007 ; http://n2t.net/addgene:85007 ; RRID:Addgene_85007)
For your References section:Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Luo W, Mizuno H, Iwata R, Nakazawa S, Yasuda K, Itohara S, Iwasato T. Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747. 10.1038/srep35747 PubMed 27775045