-
PurposeFor the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo
-
Depositing Lab
-
Depositing OrganizationNational Institute of Genetics
Located in Japan -
Citations
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69138 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 69138-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 69138-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepDisplay
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4339
- Total vector size (bp) 10266
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCAG-loxP-stop-loxP-RFP-ires-tTA-WPRE
-
Alt nameCAG-LSL-RFP-ires-tTA-WPRE
-
SpeciesG. gallus (chicken); CMV, P1 phage, SV40 , Entacmaea quadricolor, Encephalomyocarditis virus, E.coli, HSV, woodchuck hepatitis virus
-
Insert Size (bp)5927
-
Tag
/ Fusion Protein
- none
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer NA
- (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 69138-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 69138-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova) was a gift from Takuji Iwasato (Addgene plasmid # 69138 ; http://n2t.net/addgene:69138 ; RRID:Addgene_69138) -
For your References section:
NMDAR-regulated dynamics of layer 4 neuronal dendrites during thalamocortical reorganization in neonates. Mizuno H, Luo W, Tarusawa E, Saito YM, Sato T, Yoshimura Y, Itohara S, Iwasato T. Neuron. 2014 Apr 16;82(2):365-79. doi: 10.1016/j.neuron.2014.02.026. Epub 2014 Mar 27. 10.1016/j.neuron.2014.02.026 PubMed 24685175
