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pGL4.23 WT 2014 EBOV + Mucin-Like Domain
(Plasmid #85794)

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This material is available to academics and nonprofits only. Currently unavailable outside the U.S.
Item Catalog # Description Quantity Price (USD)
Plasmid 85794 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 85794-D50 50 μg of DNA in Tris buffer $495
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DNA 85794-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pGL4.23
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2031
  • Total vector size (bp) 5198
  • Modifications to backbone
    CMV IE promoter/enhancer inserted upstream of minimal promoter
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Makona Ebola Virus Glycoprotein
  • Alt name
    EBOV GP
  • Alt name
    EBOV Makona GP
  • Species
    Zaire ebolavirus - Makona
  • Insert Size (bp)
    2031
  • GenBank ID
    KJ660346.2 AHX24649.2
  • Promoter CMV IE

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV F
  • 3′ sequencing primer SV40 pA R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL4.23 WT 2014 EBOV + Mucin-Like Domain was a gift from Jeremy Luban & Pardis Sabeti (Addgene plasmid # 85794 ; http://n2t.net/addgene:85794 ; RRID:Addgene_85794)

  • For your References section:

    Ebola Virus Glycoprotein with Increased Infectivity Dominated the 2013-2016 Epidemic. Diehl WE, Lin AE, Grubaugh ND, Carvalho LM, Kim K, Kyawe PP, McCauley SM, Donnard E, Kucukural A, McDonel P, Schaffner SF, Garber M, Rambaut A, Andersen KG, Sabeti PC, Luban J. Cell. 2016 Nov 3;167(4):1088-1098.e6. doi: 10.1016/j.cell.2016.10.014. 10.1016/j.cell.2016.10.014 PubMed 27814506
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