hFLT1 delta CTD
(Plasmid
#86036)
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Purposehuman VEGFR1 with deletion of the entire intracellular domain
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Depositing Lab
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Depositing OrganizationUniversity of Iowa
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86036 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 86036-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 86036-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCMV6 Entry
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Backbone manufacturerOrigene
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman Flt1 delta CTD
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SpeciesH. sapiens (human)
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Insert Size (bp)2567
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Mutationdeta CTD
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Entrez GeneFLT1 (a.k.a. FLT, FLT-1, VEGFR-1, VEGFR1)
- Promoter CMV
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Tags
/ Fusion Proteins
- HA (N terminal on backbone)
- FLAG (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGH-PA-R
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 86036-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 86036-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hFLT1 delta CTD was a gift from Christie Thomas (Addgene plasmid # 86036 ; http://n2t.net/addgene:86036 ; RRID:Addgene_86036) -
For your References section:
N-terminal cleavage and release of the ectodomain of Flt1 is mediated via ADAM10 and ADAM 17 and regulated by VEGFR2 and the Flt1 intracellular domain. Raikwar NS, Liu KZ, Thomas CP. PLoS One. 2014 Nov 11;9(11):e112794. doi: 10.1371/journal.pone.0112794. eCollection 2014. PONE-D-14-21798 [pii] PubMed 25387128
