hNedd4-2 CS
(Plasmid
#86041)
-
Purposefull length NeddL3 with C to S mutation with His Xpress tag
-
Depositing Lab
-
Depositing OrganizationUniversity of Iowa
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86041 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 86041-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 86041-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepcDNA4 HisMax
-
Backbone manufacturerthermofisher
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namehNeddL3 with C to S mutation in HECT domain
-
Alt nameNM_001144967.2
-
SpeciesH. sapiens (human)
-
Insert Size (bp)3035
-
MutationC942S mutation in HECT domain
-
Entrez GeneNEDD4 (a.k.a. NEDD4-1, RPF1)
- Promoter CMV
-
Tag
/ Fusion Protein
- His and Xpress (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-Rev
- (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 86041-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 86041-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
hNedd4-2 CS was a gift from Christie Thomas (Addgene plasmid # 86041 ; http://n2t.net/addgene:86041 ; RRID:Addgene_86041) -
For your References section:
Nedd4-2 interacts with occludin to inhibit tight junction formation and enhance paracellular conductance in collecting duct epithelia. Raikwar NS, Vandewalle A, Thomas CP. Am J Physiol Renal Physiol. 2010 Aug;299(2):F436-44. doi: 10.1152/ajprenal.00674.2009. Epub 2010 May 26. 10.1152/ajprenal.00674.2009 PubMed 20504882
