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Human ICAM-2
(Plasmid #8634)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8634 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 8634-D50 50 μg of DNA in Tris buffer $495
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DNA 8634-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCDM8
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4356
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Unknown
  • Growth instructions
    The host strain is MC1061/P3 and the bacteria should be cultured in 12.5ug/mL ampicillin and 16ug/mL tetracycline. (supF)
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ICAM-2
  • Alt name
    CD102
  • Alt name
    pCDIC2.27
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1100
  • GenBank ID
    NM_000873
  • Entrez Gene
    ICAM2 (a.k.a. CD102)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This clone will direct the expression of functional human ICAM-2 in a variety of cell types. This sequence has been deposited in Genbank with accession number X15606. Also see ref: Nature 329, 840-842 (1987)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human ICAM-2 was a gift from Timothy Springer (Addgene plasmid # 8634 ; http://n2t.net/addgene:8634 ; RRID:Addgene_8634)

  • For your References section:

    Functional cloning of ICAM-2, a cell adhesion ligand for LFA-1 homologous to ICAM-1. Staunton DE, Dustin ML, Springer TA. Nature 1989 May 4;339(6219):61-4. 10.1038/339061a0 PubMed 2497351
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