|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8632||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4356
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Tetracycline, 100 & 10 μg/mL
Growth instructionsThe host strain is MC1061/P3 and the bacteria should be cultured in 12.5ug/mL ampicillin and 16ug/mL tetracycline. (supF)
SpeciesH. sapiens (human)
Insert Size (bp)1800
Entrez GeneICAM1 (a.k.a. BB2, CD54, P3.58)
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This clone will direct the expression of functional human ICAM-1 in a variety of cell types. This sequence has been deposited in Genbank with accession number J03132. Also see refs: Nature 329, 840-842 (1987) and Nature 339, 61-64 (1989)
Please use either GenBank IDs NM_000201.1 or J03132.1 as a refence sequence for the ICAM-1 insert in this plasmid. GenBank ID NM_000201.2 (updated after this plasmid was created) is not accurate for comparison.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Human ICAM-1 was a gift from Timothy Springer (Addgene plasmid # 8632 ; http://n2t.net/addgene:8632 ; RRID:Addgene_8632)
For your References section:Primary structure of ICAM-1 demonstrates interaction between members of the immunoglobulin and integrin supergene families. Staunton DE, Marlin SD, Stratowa C, Dustin ML, Springer TA. Cell 1988 Mar 25;52(6):925-33. 10.1016/0092-8674(88)90434-5 PubMed 3349522