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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8630 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAPRM8
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Backbone manufacturern/a
- Backbone size w/o insert (bp) 5100
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLFA1 alpha
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Alt nameCD11a
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Alt namepCDL1
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SpeciesH. sapiens (human)
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Insert Size (bp)4322
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GenBank IDNM_002209
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Entrez GeneITGAL (a.k.a. CD11A, LFA-1, LFA1A)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Human LFA-1 alpha was a gift from Timothy Springer (Addgene plasmid # 8630 ; http://n2t.net/addgene:8630 ; RRID:Addgene_8630) -
For your References section:
Primary structure of the leukocyte function-associated molecule-1 alpha subunit: an integrin with an embedded domain defining a protein superfamily. Larson RS, Corbi AL, Berman L, Springer T. J Cell Biol 1989 Feb;108(2):703-12. 10.1083/jcb.108.2.703 PubMed 2537322
Map uploaded by the depositor.
