|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8633||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4356
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Tetracycline
Growth instructionsThe host strain is MC1061/P3 and the bacteria should be cultured in 12.5ug/mL ampicillin and 16ug/mL tetracycline. (supF)
Gene/Insert nameHuman p150,95
SpeciesH. sapiens (human)
Insert Size (bp)4500
Entrez GeneITGAX (a.k.a. CD11C, SLEB6)
- Cloning method Restriction Enzyme
- 5′ cloning site BstXI (not destroyed)
- 3′ cloning site BstXI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
This clone will direct the expression of functional p150,95 upon cotransfection with the beta2 subunit in a variety of cell types. This sequence has been deposited in Genbank with accession number Y00093. Also see refs: Nature 329, 840-842 (1987) and J of Immunology 146, 648-655 (1991).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Human p150,95 was a gift from Timothy Springer (Addgene plasmid # 8633 ; http://n2t.net/addgene:8633 ; RRID:Addgene_8633)
For your References section:cDNA cloning and complete primary structure of the alpha subunit of a leukocyte adhesion glycoprotein, p150,95. Corbi AL, Miller LJ, O'Connor K, Larson RS, Springer TA. EMBO J 1987 Dec 20;6(13):4023-8. PubMed 3327687
Map uploaded by the depositor.