Integrin beta 2 - mYFP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8638||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepEYFP N1
- Backbone size w/o insert (bp) 4900
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert nameIntegrin beta 2
SpeciesH. sapiens (human)
Insert Size (bp)3500
Mutationstop codon of beta2 was replaced with AgeI restriction site. Leu221 of YFP was replaced with lysine by PCR to generate monomeric YFP.
Entrez GeneITGB2 (a.k.a. CD18, LAD, LCAMB, LFA-1, MAC-1, MF17, MFI7)
/ Fusion Protein
- YFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site AgeI (not destroyed)
- 5′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Integrin beta 2 - mYFP was a gift from Timothy Springer (Addgene plasmid # 8638 ; http://n2t.net/addgene:8638 ; RRID:Addgene_8638)
For your References section:Bidirectional transmembrane signaling by cytoplasmic domain separation in integrins. Kim M, Carman CV, Springer TA. Science 2003 Sep 19;301(5640):1720-5. 10.1126/science.1084174 PubMed 14500982