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Integrin beta 2 - mYFP
(Plasmid #8638)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8638 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 8638-D50 50 μg of DNA in Tris buffer $495
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DNA 8638-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEYFP N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Integrin beta 2
  • Alt name
    CD18
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3500
  • Mutation
    stop codon of beta2 was replaced with AgeI restriction site. Leu221 of YFP was replaced with lysine by PCR to generate monomeric YFP.
  • Entrez Gene
    ITGB2 (a.k.a. CD18, LAD, LCAMB, LFA-1, MAC-1, MF17, MFI7)
  • Tag / Fusion Protein
    • YFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Integrin beta 2 - mYFP was a gift from Timothy Springer (Addgene plasmid # 8638 ; http://n2t.net/addgene:8638 ; RRID:Addgene_8638)

  • For your References section:

    Bidirectional transmembrane signaling by cytoplasmic domain separation in integrins. Kim M, Carman CV, Springer TA. Science 2003 Sep 19;301(5640):1720-5. 10.1126/science.1084174 PubMed 14500982

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