pEGFP-C1-AR V7
(Plasmid #86430)

• Purpose: Fluorescent human androgen-receptor splice variant 7, lacking the ligand-binding domain (fused to EGFP)
• Depositing Lab: Michael Mancini
• Depositing Organization: Baylor College of Medicine
  Located in the United States of America
• Publication: pEGFP-C1-AR V7 (unpublished)

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech (Takara)
• Backbone size w/o insert (bp): 4731
• Total vector size (bp): 6642
• Modifications to backbone: none
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Sequence-verify the poly-glutamine expansion when preparing new plasmid preps.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Androgen receptor (AR)
• Species: H. sapiens (human)
• Insert Size (bp): 1932
• Mutation: Alternative splice variant 7 (alteration/deletion of the C-terminus)
• GenBank ID: NM_000044.3
• Entrez Gene: AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XmaI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Co-author Marco Marcelli, Baylor College of Medicine

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry