FKBP*-PTEN
(Plasmid
#86781)
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PurposeFusion of PTEN with a modified human FKBP variant harboring the F36V and L106P point mutations (FKBP*) leads to degradation of the fusion protein.
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Depositing Lab
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Depositing OrganizationCharite Universitaetsmedizin Berlin
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86781 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 86781-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 86781-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCAX
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePTEN
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Alt namephosphatase and tensin homolog
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SpeciesH. sapiens (human)
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Entrez GenePTEN (a.k.a. 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, PTENgama, TEP1)
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Tags
/ Fusion Proteins
- FKBP* (F36V and L106P) (N terminal on insert)
- linker (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pCAG
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 86781-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 86781-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FKBP*-PTEN was a gift from Britta Eickholt (Addgene plasmid # 86781 ; http://n2t.net/addgene:86781 ; RRID:Addgene_86781) -
For your References section:
Engineering FKBP-Based Destabilizing Domains to Build Sophisticated Protein Regulation Systems. An W, Jackson RE, Hunter P, Gogel S, van Diepen M, Liu K, Meyer MP, Eickholt BJ. PLoS One. 2015 Dec 30;10(12):e0145783. doi: 10.1371/journal.pone.0145783. eCollection 2015. PONE-D-15-39012 [pii] PubMed 26717575
