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Depositing Lab
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Depositing OrganizationRockefeller University
Located in the United States of America -
Publication
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Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8688 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 8688-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 8688-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepTATA TK-Luc
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name4 repeated M67 sites
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Insert Size (bp)98
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AccI (not destroyed)
- 3′ cloning site BamHI (destroyed during cloning)
- 5′ sequencing primer lucNrev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
To obtain p4xM67-tk-Luc, an oligonucleotide containing four copies of the sequence GGTTCCCGTAAATGCATCA (TTCCCGTAA is the STAT-binding site) was introduced in the AccI-BamHI sites of pTATA-tk-Luc upstream of the minimal promoter. Diagnostic digest is EcoRI (3.6kb, 1.7kb, 0.6kb).
DNA (Catalog # 8688-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 8688-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
4xM67 pTATA TK-Luc was a gift from Jim Darnell (Addgene plasmid # 8688 ; http://n2t.net/addgene:8688 ; RRID:Addgene_8688) -
For your References section:
A single amino acid substitution in the v-Eyk intracellular domain results in activation of Stat3 and enhances cellular transformation. Besser D, Bromberg JF, Darnell JE Jr, Hanafusa H. Mol Cell Biol 1999 Feb;19(2):1401-9. PubMed 9891073
Map uploaded by the depositor.
