p28-2iDMD-puro
(Plasmid #88895)

• Purpose: 28°C full-length DMD luc-puro-red plasmid. Co-expresses human DMD, firefly luciferase, puroR and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites, but *cannot* be grown at 37°C.
• Depositing Lab: Michele Calos
• Depositing Organization: Stanford University
  Located in the United States of America
• Publication: Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/biot.201600477.

Backbone

• Vector backbone: p28
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 25 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Growth instructions: We recommend growth at 28°C. Depositor recommends DH10B, XL1 blue or NEB's DH10-beta for growth. DH5alpha should not be used as it does not yield good transformation results
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Dystrophin
• Alt name: DMD
• Species: H. sapiens (human)
• Insert Size (bp): 11262
• Entrez Gene: DMD (a.k.a. BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, MRX85)

Gene/Insert 2

• Gene/Insert name: luciferase
• Alt name: luc
• Species: Firefly
• Insert Size (bp): 1647

Gene/Insert 3

• Gene/Insert name: puromycin resistance enzyme
• Alt name: puroR
• Insert Size (bp): 594
• Mutation: codon optimized

Gene/Insert 4

• Gene/Insert name: mCherry
• Alt name: red
• Insert Size (bp): 705

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid *cannot* be grown at 37°C

DNA (Catalog # 88895-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 88895-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.