pLv-HSA-uDys/eGFP
(Plasmid #26810)

• Depositing Lab: Jeffrey Chamberlain
• Publication: Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-17.

Backbone

• Vector backbone: pRRL-cPPT-X-PRE-SIN
• Backbone size w/o insert (bp): 6656
• Vector type: Lentiviral ; lentiviral plasmid backbone from L. Naldini

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: dystrophin
• Species: H. sapiens (human)
• Insert Size (bp): 7233
• Mutation: deleted R4-R23 deleted exons 71-78
• Entrez Gene: DMD (a.k.a. BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, MRX85)
• Tag / Fusion Protein:
  • eGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (unknown if destroyed)
• 3′ cloning site: PacI (unknown if destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: EGFP-N

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Human (alpha) Skeletal Actin (HSA) promotor
Vp intron