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YFP-DAT R60A
(Plasmid #90247)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 90247 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEYFP-C1
  • Backbone size w/o insert (bp) 4731
  • Total vector size (bp) 6589
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DAT (dopamine transporter)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1863
  • Mutation
    mutated R60 to A in human DAT cDNA
  • Entrez Gene
    SLC6A3 (a.k.a. DAT, DAT1, PKDYS, PKDYS1)
  • Promoter CMV

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YFP-DAT R60A was a gift from Alexander Sorkin (Addgene plasmid # 90247 ; http://n2t.net/addgene:90247 ; RRID:Addgene_90247)
  • For your References section:

    Targeting of dopamine transporter to filopodia requires an outward-facing conformation of the transporter. Ma S, Cheng MH, Guthrie DA, Newman AH, Bahar I, Sorkin A. Sci Rep. 2017 Jul 14;7(1):5399. doi: 10.1038/s41598-017-05637-x. 10.1038/s41598-017-05637-x PubMed 28710426