RFP-HA-DAT
(Plasmid #90265)

• Purpose: Expresses RFP-HA-DAT in mammalian cells
• Depositing Lab: Alexander Sorkin
• Publication: Caltagarone et al Mol Cell Neurosci. 2015 Sep;68:120-30. doi: 10.1016/j.mcn.2015.04.005. Epub 2015 Apr 30.

Backbone

• Vector backbone: tagRFP-C1
• Backbone size w/o insert (bp): 4725
• Total vector size (bp): 6583
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: DAT (dopamine transporter)
• Species: H. sapiens (human)
• Insert Size (bp): 1863
• Mutation: inserted HA tag (see YFP-HA-DAT)
• Entrez Gene: SLC6A3 (a.k.a. DAT, DAT1, PKDYS, PKDYS1)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: SmaI (not destroyed)
• 5′ sequencing primer: NO
• 3′ sequencing primer: NO

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry