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(Plasmid #9044)


Item Catalog # Description Quantity Price (USD)
Plasmid 9044 Standard format: Plasmid sent in bacteria as agar stab 1 $75
Cloning Grade DNA 2 µg of cloning grade DNA in Tris buffer 1 $95

This material is available to academics and nonprofits only.


  • Vector backbone
    modified pMSCV 2.2
  • Backbone size (bp) 6500
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Tag / Fusion Protein
    • IRES GFP (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Excised IRES hGFP-3M from pCITE IRES hGFP-3M with EcoRI/SalI digest. Ligated into MSCV2.2 following EcorI/SalI digest of vector. Lost puro resistance gene (pac) in process. Luk Van Parijs et al., Immunity 11(3): 281.

Also see Addgene Plasmid #12282, pMIG-w, which is a variant of pMIG containing a WRE element. The WRE element increases export to the cytoplasm and boosts protein expression by 2-10 fold.

Please see plasmid 20672: MSCV-IRES-GFP for sequence reference.

Information for Cloning Grade DNA (Catalog # ( Back to top )


Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.


  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $95 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIG was a gift from William Hahn (Addgene plasmid # 9044 ; ; RRID:Addgene_9044)