|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||9044||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonemodified pMSCV 2.2
- Backbone size (bp) 6500
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Alt nameMSCV IRES GFP
/ Fusion Protein
- IRES GFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer pLXSN 5' (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
Excised IRES hGFP-3M from pCITE IRES hGFP-3M with EcoRI/SalI digest. Ligated into MSCV2.2 following EcorI/SalI digest of vector. Lost puro resistance gene (pac) in process. Luk Van Parijs et al., Immunity 11(3): 281.
Also see Addgene Plasmid #12282, pMIG-w, which is a variant of pMIG containing a WRE element. The WRE element increases export to the cytoplasm and boosts protein expression by 2-10 fold.
Please see plasmid 20672: MSCV-IRES-GFP for sequence reference.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMIG was a gift from William Hahn (Addgene plasmid # 9044 ; http://n2t.net/addgene:9044 ; RRID:Addgene_9044)