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(Plasmid #91776)


Item Catalog # Description Quantity Price (USD)
Plasmid 91776 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 8054
  • Total vector size (bp) 8091
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
    Human Histone H2B / eGFP
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    H2BC11 (a.k.a. H2B/r, H2BFR, H2BJ, HIST1H2BJ)
  • Promoter EF1a
  • Tag / Fusion Protein
    • eGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer atcatgaattcgtttgtgaacgacattttcgagcg
  • 3′ sequencing primer atcgatGTCGCGGCCGCTTTACTTGTACA
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    We used the H2B-GFP plasmid from Geoff Wahl (Addgene plasmid # 11680) as donor vector
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The HIV-H2BeGFP plasmid was cloned by replacing mRFP in HIV-H2B-mRFP (Bryan Welm & Zena Werb Addgene plasmid # 18982 ) with H2BeGFP from H2B-GFP plasmid (Geoff Wahl Addgene plasmid # 11680) to generate a bicistronic lentiviral vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHIV-H2B-eGFP was a gift from Maria Pia Cosma (Addgene plasmid # 91776 ; ; RRID:Addgene_91776)
  • For your References section:

    Mesenchymal stem cells generate distinct functional hybrids in vitro via cell fusion or entosis. Sottile F, Aulicino F, Theka I, Cosma MP. Sci Rep. 2016 Nov 9;6:36863. doi: 10.1038/srep36863. 10.1038/srep36863 PubMed 27827439