|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18982||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 8046
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth instructionsGrowth in Stbl3 bacteria is preferred.
Copy numberHigh Copy
Gene/Insert nameHistone H2B monomeric red fluorescent protein
Insert Size (bp)1065
- Cloning method Restriction Enzyme
- 5′ sequencing primer 5â€™-TGGAATTTGCCCTTTTTGAG-3â€™
- 3′ sequencing primer 5â€™-AGGAACTGCTTCCTTCACGA-3â€™ (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The HIV-H2BmRFP plasmid was cloned by replacing Zsgreen in HIV-Zsgreen with H2BmRFP to generate a bicistronic lentiviral vector. cDNA can be cloned into the multiple cloning site (HpaI, XbaI, SmaI) in order to coexpress the inserted gene with H2BmRFP. Gerrit Dijkgraaf in Zena Werb's lab cloned this virus.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHIV-H2BmRFP was a gift from Bryan Welm & Zena Werb (Addgene plasmid # 18982 ; http://n2t.net/addgene:18982 ; RRID:Addgene_18982)
For your References section:Lentiviral transduction of mammary stem cells for analysis of gene function during development and cancer. Welm BE, Dijkgraaf GJ, Bledau AS, Welm AL, Werb Z. Cell Stem Cell. 2008 Jan 10. 2(1):90-102. 10.1016/j.stem.2007.10.002 PubMed 18371425