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pHIV-H2BmRFP
(Plasmid #18982)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18982 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    HIV-Zsgreen
  • Backbone size w/o insert (bp) 8046
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Growth in Stbl3 bacteria is preferred.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Histone H2B monomeric red fluorescent protein
  • Alt name
    H2BmRFP
  • Alt name
    H2B-mRFP
  • Species
    Discosoma coral
  • Insert Size (bp)
    1065

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer 5’-TGGAATTTGCCCTTTTTGAG-3’
  • 3′ sequencing primer 5’-AGGAACTGCTTCCTTCACGA-3’
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We obtained H2BmRFP from Sean Megason and Scott Fraser at the California Institute of Technology. The mRFP protein was originally described in Campbell, E.R., et al. PNAS
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

The HIV-H2BmRFP plasmid was cloned by replacing Zsgreen in HIV-Zsgreen with H2BmRFP to generate a bicistronic lentiviral vector. cDNA can be cloned into the multiple cloning site (HpaI, XbaI, SmaI) in order to coexpress the inserted gene with H2BmRFP. Gerrit Dijkgraaf in Zena Werb's lab cloned this virus.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHIV-H2BmRFP was a gift from Bryan Welm & Zena Werb (Addgene plasmid # 18982 ; http://n2t.net/addgene:18982 ; RRID:Addgene_18982)
  • For your References section:

    Lentiviral transduction of mammary stem cells for analysis of gene function during development and cancer. Welm BE, Dijkgraaf GJ, Bledau AS, Welm AL, Werb Z. Cell Stem Cell. 2008 Jan 10. 2(1):90-102. 10.1016/j.stem.2007.10.002 PubMed 18371425