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pHIV-dTomato
(Plasmid #21374)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21374 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Growth in Stbl3 is preferred
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer 5'-TGGAATTTGCCCTTTTTGAG-3'
  • 3′ sequencing primer 5'-AGGAACTGCTTCCTTCACGA-3'
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The HIV-dTomato plasmid encodes a self-inactivating lentiviral plasmid that was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and EGFP. A cDNA can be cloned into the MCS (NotI, EcoRI, HpaI, XbaI, SmaI, and BamHI sites) to enable bicistronic expression with dTomato. This construct was made in Zena Werb's laboratory and has not been published. Note: This plasmid contains dTomato and not the tandem conjugate TdTomato.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHIV-dTomato was a gift from Bryan Welm (Addgene plasmid # 21374)