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pAAV hSyn-Flex RG-cerulean
(Plasmid #98221)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 98221 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

This item is currently unavailable outside the US without additional regulatory approval.
A non-refundable shipping export licensing fee of $85 is required to cover Addgene’s additional processing costs.

Backbone

  • Vector backbone
    pAAV Ef1a flex C-RG
  • Backbone size w/o insert (bp) 4223
  • Total vector size (bp) 6590
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cerulean-E2A-RG
  • Alt name
    rabies virus glycoprotein
  • Species
    rabies virus
  • Insert Size (bp)
    2367
  • Promoter human Synapsin
  • Tag / Fusion Protein
    • cerulean (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer gaccatctgcgctgcggcg
  • 3′ sequencing primer CAAGGCTGGTGGGCACTGG
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    I used the Addgene plasmid 26197 to originally amplify the RG portion of the insert
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV hSyn-Flex RG-cerulean was a gift from Troy Margrie (Addgene plasmid # 98221 ; http://n2t.net/addgene:98221 ; RRID:Addgene_98221)